Most fungi grow more slowly than bacteria. They grow on usual bacteriological culture media at a temperature of 20-30°C. High sugar content in acidic media (pH 5.6) is tolerant to molds. Generally, glucose and peptone content media are useful for the isolation of molds and some yeast. Some other well-known media such as solid Sabouraud agar media is used for the culture of fungi which contain maltose and peptone with less agar content. Media is acidic with pH 5.5 which inhibits bacterial growth. Potato Dextrose agar, brain heart infusion, and Czapekdox agar media are also used to isolate fungi. Yeast grows in colonies whereas filamentous fungi grow as a mycelium. Yeast forms colony: Example: yeast, Cryptococcus neoformans. Yeast-like colony: Example: Candida albicans. Hyphomycete form colony: Example: Dermatophytes. Saprophytic fungi can grow in the media containing chloramphenicol and cyclohexamid. Papanicolaou stain is good for the initial differentiation of dimorphic fungi. Most of the fungi are gram-positive but Nocardia and actinomyces are gram variable.