Quantitative Measurement of Bacterial Growth

After incubation, the growth of microorganisms is detected, followed by counting. There are several techniques involved in counting microbes. Direct counting techniques are used. The oldest of these is microscopy which involves m…

Preservation Methods For Pure Cultures

Preservation is a process of maintaining pure culture for extended periods in a viable condition without any genetic changes. Once a pure culture has been obtained from the mixture, the microbes may be maintained in the laborator…

Isolation of Pure Culture

A pure culture is usually derived from a mixed culture by transferring a small sample into a new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample seve…

Bacterial Growth Curve

The bacterial growth curve has four distinct phases viz. Lag phase, Log phase, Stationary phase, and Decline phase (Fig). (a) Lag Phase: The bacteria first undergo a period of acclimatization when they are inoculated into the …

Physical Factors Affecting Bacterial Growth

Some of the important factors affecting bacterial growth are: 1. Nutrition concentration 2. Temperature 3. Gaseous concentration 4. pH 5. Ions and salt concentration 6. Water 1. Nutrient concentration: Bacterial …

Culture Media

Culture media are the base where microorganisms can grow with the help of nutrients and under the influence of physical growth parameters. All microorganisms cannot grow in a single culture medium and in fact, many cannot grow in…

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