General Procedure for Cell Culture

Fig. 1: Steps of animal cell culture (a) Requirements: Vertical Laminar air flow, Incubator, Refrigerator, Microscope, Tissue culture ware. (b) Temperature: The temperature is set at the same as the body temperature of th…

Growth of Animal Cells In Culture

The removal of cells from animals and their subsequent growth in a favorable artificial environment is called animal cell culture. Animal cells are more difficult to culture than microorganisms because animal cells require many m…

Evaluation of Microbial Stability of Formulations

Stability is defined as the extent to which a pharmaceutical product retains, within specified limits and throughout its storage period and use, i.e. its shelf life, the same properties and characteristics that are possessed at t…

Assessment of Microbial Contamination And Spoilage

Early indications for microbial spoilage are gas production, pH changes, and organoleptic changes like smell, sour, fishy amines, bad eggs, earthy taste, bitter, etc. Microbial polymerization of sugars and surfactant molecules ca…

Sources And Types Of Microbial Contaminants

Most of the raw materials for pharmaceutical products support microbial growth based on the nutritive properties and moisture contents. Microbiological contaminants are infectious materials, such as bacteria, mold, fungi, and vir…

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